Contact an Account ManagerSeparation identification and quantitation of ceramides in human cancer cells
Summary
Type: HPLC
Application #: 117630
Detection: MS detection wit a Z-spray electrospray ionization source
Conditions: Eluents: A: water-acetonitrile-2-propanol (8:1:1, v/v/v), B: acetonitrile-2-propanol (9:1, v/v)
Gradient: 0 min 35:65 (A-B), 2 min 35:65 (A-B), 7 min 10:90 (A-B), 13 min 10:90 (A-B) 15 min 0:100 (A-B), 16 min 35:65 (A-B), 18 min 35:65 (A-B)
Injection: 6 μl
Flow rate: 0,450 ml/min
Authors: Fillet M Van Heugen J-C Servais AC De Graeve J Crommen J
Source: Journal of Chromatography A 949 (2002) 225-233
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